il 17 ifn γ Search Results


human ifn  (Cellular Technology Ltd)
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Cellular Technology Ltd human ifn
Human Ifn, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine ifn  (Cellular Technology Ltd)
96
Cellular Technology Ltd murine ifn
Murine Ifn, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 17 immunospot kit  (Cellular Technology Ltd)
96
Cellular Technology Ltd il 17 immunospot kit
PBMC from 45 donors was tested in ELISPOT assays without adding HCMV (antigen) or in the presence of UV-inactivated HCMV virions. The assay was performed as described in Materials and Methods with 250,000 PBMC per well and 50 μg/mL of HCMV antigen. ( A ) Representative well images of one donor for medium control and antigen-specific IFN-γ, IL-2, IL-4, <t>and</t> <t>IL-17</t> assays are shown at the time point of peak secretion; ( B ) PBMC was stimulated with HCMV in ELISPOT assays for 24 h, 48 h, and 72 h. IFN-γ, IL-2, IL-4 <t>and</t> <t>IL-17</t> responses exhibited as spot forming units (SFU) were recorded at each time point. ( n = 3) ( C ) Maximal number of IFN-γ spots was observed at 24 h. At later time points (72 h) the ELISPOT image was over developed.
Il 17 Immunospot Kit, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
il 17 immunospot kit - by Bioz Stars, 2026-02
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mrna expression for il-17 and ifnγ  (Perio Products Ltd)
90
Perio Products Ltd mrna expression for il-17 and ifnγ
PBMC from 45 donors was tested in ELISPOT assays without adding HCMV (antigen) or in the presence of UV-inactivated HCMV virions. The assay was performed as described in Materials and Methods with 250,000 PBMC per well and 50 μg/mL of HCMV antigen. ( A ) Representative well images of one donor for medium control and antigen-specific IFN-γ, IL-2, IL-4, <t>and</t> <t>IL-17</t> assays are shown at the time point of peak secretion; ( B ) PBMC was stimulated with HCMV in ELISPOT assays for 24 h, 48 h, and 72 h. IFN-γ, IL-2, IL-4 <t>and</t> <t>IL-17</t> responses exhibited as spot forming units (SFU) were recorded at each time point. ( n = 3) ( C ) Maximal number of IFN-γ spots was observed at 24 h. At later time points (72 h) the ELISPOT image was over developed.
Mrna Expression For Il 17 And Ifnγ, supplied by Perio Products Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits il-2, il-4, il-6, il-17, tnf-α ifn-γ  (Jingmei Biotech Co Ltd)
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Jingmei Biotech Co Ltd elisa kits il-2, il-4, il-6, il-17, tnf-α ifn-γ
PBMC from 45 donors was tested in ELISPOT assays without adding HCMV (antigen) or in the presence of UV-inactivated HCMV virions. The assay was performed as described in Materials and Methods with 250,000 PBMC per well and 50 μg/mL of HCMV antigen. ( A ) Representative well images of one donor for medium control and antigen-specific IFN-γ, IL-2, IL-4, <t>and</t> <t>IL-17</t> assays are shown at the time point of peak secretion; ( B ) PBMC was stimulated with HCMV in ELISPOT assays for 24 h, 48 h, and 72 h. IFN-γ, IL-2, IL-4 <t>and</t> <t>IL-17</t> responses exhibited as spot forming units (SFU) were recorded at each time point. ( n = 3) ( C ) Maximal number of IFN-γ spots was observed at 24 h. At later time points (72 h) the ELISPOT image was over developed.
Elisa Kits Il 2, Il 4, Il 6, Il 17, Tnf α Ifn γ, supplied by Jingmei Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ifn  (Cellular Technology Ltd)
96
Cellular Technology Ltd ifn
Effects of tumour necrosis factor (TNF)-α blockade on the frequencies of antigen-specific Th1/Th17 cells in spleen and central nervous system (CNS). (a) Results of enzyme-linked immunospot (elispot) assays in phosphate-buffered saline (PBS) (n = 12)-, Humira® (n = 8)- and Enbrel®-treated mice (n = 8) for interferon <t>(IFN)-γ</t> (**P < 0·01, rank sum test) and <t>interleukin</t> <t>(IL-17</t> (*P < 0·05, rank sum test) in the spleen. (b) Results <t>of</t> <t>IFN-γ/IL-17</t> elispot assays in PBS (n = 13)-, Humira® (n = 5)- and Enbrel®-treated mice (n = 8 IFN-γ; n = <t>7</t> <t>IL-17)</t> in the spinal cord.
Ifn, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ifn - by Bioz Stars, 2026-02
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Image Search Results


PBMC from 45 donors was tested in ELISPOT assays without adding HCMV (antigen) or in the presence of UV-inactivated HCMV virions. The assay was performed as described in Materials and Methods with 250,000 PBMC per well and 50 μg/mL of HCMV antigen. ( A ) Representative well images of one donor for medium control and antigen-specific IFN-γ, IL-2, IL-4, and IL-17 assays are shown at the time point of peak secretion; ( B ) PBMC was stimulated with HCMV in ELISPOT assays for 24 h, 48 h, and 72 h. IFN-γ, IL-2, IL-4 and IL-17 responses exhibited as spot forming units (SFU) were recorded at each time point. ( n = 3) ( C ) Maximal number of IFN-γ spots was observed at 24 h. At later time points (72 h) the ELISPOT image was over developed.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: PBMC from 45 donors was tested in ELISPOT assays without adding HCMV (antigen) or in the presence of UV-inactivated HCMV virions. The assay was performed as described in Materials and Methods with 250,000 PBMC per well and 50 μg/mL of HCMV antigen. ( A ) Representative well images of one donor for medium control and antigen-specific IFN-γ, IL-2, IL-4, and IL-17 assays are shown at the time point of peak secretion; ( B ) PBMC was stimulated with HCMV in ELISPOT assays for 24 h, 48 h, and 72 h. IFN-γ, IL-2, IL-4 and IL-17 responses exhibited as spot forming units (SFU) were recorded at each time point. ( n = 3) ( C ) Maximal number of IFN-γ spots was observed at 24 h. At later time points (72 h) the ELISPOT image was over developed.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Enzyme-linked Immunospot

The spot size distribution for different cytokines follow Log Normal distribution. The experimental size distribution of typical recall responses are shown as histograms for the specific cytokines (IFN-g, IL-2, IL-4, and IL-17) with the theoretical Log Normal distribution curve overlaid in red. To test for normality, Kolmogorov-Smirnov goodness of fit was used.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: The spot size distribution for different cytokines follow Log Normal distribution. The experimental size distribution of typical recall responses are shown as histograms for the specific cytokines (IFN-g, IL-2, IL-4, and IL-17) with the theoretical Log Normal distribution curve overlaid in red. To test for normality, Kolmogorov-Smirnov goodness of fit was used.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques:

HCMV induced recall response is produced by CD4 cells for all cytokines. Unseparated PBMC, CD8 depleted PBMC, and CD4 depleted PBMC (2.5 × 10 5 cells each) were tested in human ELISPOT assays to detect the secretion of IFN-γ, IL-2, IL-4, and IL-17 at their appropriate time points as described in . CD4 and CD8 depletions were carried out by magnetic bead separations. ( A ) FACS analysis of the unseparated, CD8-depleted and CD4-depleted PBMC populations is shown; ( B ) Representative well images from one donor for the unseparated PBMC, CD8-depleted PBMC, and CD4-depleted PBMC with IFN-γ, IL-2, IL-4, and IL-17 recall responses, following activation with HCMV antigen for 24 h, 24 h, 48 h, and 72 h respectively is shown; ( C ) The mean and SD (Standard deviation) of cytokine spot forming units (SFU) from 2.5 × 10 5 unseparated PBMC (shown as solid bars), CD8-depleted PBMC (hatched bars), and CD4-depleted PBMC (empty bars) each are shown (n = 6).

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: HCMV induced recall response is produced by CD4 cells for all cytokines. Unseparated PBMC, CD8 depleted PBMC, and CD4 depleted PBMC (2.5 × 10 5 cells each) were tested in human ELISPOT assays to detect the secretion of IFN-γ, IL-2, IL-4, and IL-17 at their appropriate time points as described in . CD4 and CD8 depletions were carried out by magnetic bead separations. ( A ) FACS analysis of the unseparated, CD8-depleted and CD4-depleted PBMC populations is shown; ( B ) Representative well images from one donor for the unseparated PBMC, CD8-depleted PBMC, and CD4-depleted PBMC with IFN-γ, IL-2, IL-4, and IL-17 recall responses, following activation with HCMV antigen for 24 h, 24 h, 48 h, and 72 h respectively is shown; ( C ) The mean and SD (Standard deviation) of cytokine spot forming units (SFU) from 2.5 × 10 5 unseparated PBMC (shown as solid bars), CD8-depleted PBMC (hatched bars), and CD4-depleted PBMC (empty bars) each are shown (n = 6).

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Produced, Enzyme-linked Immunospot, Activation Assay, Standard Deviation

ELISPOT responses for IFN-γ, IL-2, IL-4, and IL-17. PBMC from 45 donors were plated at 250,000 cells/well and 50 μg/mL of HCMV antigen was added to induce a recall response for INF-γ (blue bars), IL-2 (red bars), IL-4 (green bars), and IL-17 (purple bars) from CD4 cells. SFU for each cytokine was established. SFU greater than 100 spots per well are not shown here. Raw data is available as part of Table S1.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: ELISPOT responses for IFN-γ, IL-2, IL-4, and IL-17. PBMC from 45 donors were plated at 250,000 cells/well and 50 μg/mL of HCMV antigen was added to induce a recall response for INF-γ (blue bars), IL-2 (red bars), IL-4 (green bars), and IL-17 (purple bars) from CD4 cells. SFU for each cytokine was established. SFU greater than 100 spots per well are not shown here. Raw data is available as part of Table S1.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Enzyme-linked Immunospot

Cell number dependence of ELISPOT formation. PBMC were plated at 100,000 250,000, and 500,000 cells/well and 50 μg/mL of HCMV antigen was added to induce a recall response for INF-γ, IL-2, IL-4, and IL-17 from CD4 cells. SFU at the different PBMC numbers plated were established. Mean values and the regression coefficient (R 2 ) from four donors is shown.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: Cell number dependence of ELISPOT formation. PBMC were plated at 100,000 250,000, and 500,000 cells/well and 50 μg/mL of HCMV antigen was added to induce a recall response for INF-γ, IL-2, IL-4, and IL-17 from CD4 cells. SFU at the different PBMC numbers plated were established. Mean values and the regression coefficient (R 2 ) from four donors is shown.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Enzyme-linked Immunospot

Establishing optimal antigen dose for HCMV-specific CD4 cell stimulation. PBMC were plated at 250,000 cells per well and was stimulated with different concentrations of HCMV antigen starting from 100 μg/mL to 0.01 μg/mL. SFU recorded from IFN-γ, IL-2, IL-4, and IL-17 ELISPOT assays are shown for each of these antigen concentrations.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: Establishing optimal antigen dose for HCMV-specific CD4 cell stimulation. PBMC were plated at 250,000 cells per well and was stimulated with different concentrations of HCMV antigen starting from 100 μg/mL to 0.01 μg/mL. SFU recorded from IFN-γ, IL-2, IL-4, and IL-17 ELISPOT assays are shown for each of these antigen concentrations.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Cell Stimulation, Enzyme-linked Immunospot

Establishing cytokine signature of CD4 cells. PBMC from 45 healthy donors were tested for HCMV-induced cytokine production of IFN-γ, IL-2, IL-4, and IL-17. ( A ) The percentage of donors that generated a recall response to the HCMV antigen for each of the cytokines is shown. ( B ) The percentage of individual donors representing the various CD4 effector lineages is shown.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: Establishing cytokine signature of CD4 cells. PBMC from 45 healthy donors were tested for HCMV-induced cytokine production of IFN-γ, IL-2, IL-4, and IL-17. ( A ) The percentage of donors that generated a recall response to the HCMV antigen for each of the cytokines is shown. ( B ) The percentage of individual donors representing the various CD4 effector lineages is shown.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Generated

Longitudinal studies of donors show similarity in responses over multiple years. PBMC from six different donors were isolated at different time points over years. Some donors were tested multiple times within 1 year (Donor ID 13, 41, 44), others within 3 years (ID 43, 45) and one donor was tested over 5 years (ID 42). The dates at which PBMC was isolated is specified in the figure for each donor. PBMC isolated from each donor at the different time points, at 250,000 per well, was activated with 50 μg/mL HCMV antigen and the cytokine response for IFN-γ, IL-2, IL-4, and IL-17 was determined. Mean and SD of SFU from three repetitive wells are shown.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: Longitudinal studies of donors show similarity in responses over multiple years. PBMC from six different donors were isolated at different time points over years. Some donors were tested multiple times within 1 year (Donor ID 13, 41, 44), others within 3 years (ID 43, 45) and one donor was tested over 5 years (ID 42). The dates at which PBMC was isolated is specified in the figure for each donor. PBMC isolated from each donor at the different time points, at 250,000 per well, was activated with 50 μg/mL HCMV antigen and the cytokine response for IFN-γ, IL-2, IL-4, and IL-17 was determined. Mean and SD of SFU from three repetitive wells are shown.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Isolation

Restoration of functionality of exhausted CD4 cells with IL-7. PBMC from 40 donors were tested in an ELISPOT assay with 50 μg/mL of HCMV antigen, with and without IL-7 added to the culture. Cytokine recall response for IFN-γ, IL-2, IL-4, and IL-17 was recorded as SFU. PBMC were plated at 250,000 cells and the concentration of IL-7 was 30 ng/mL. The SFU with and without IL-7 for each of the cytokines is shown.

Journal: Viruses

Article Title: Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

doi: 10.3390/v7082828

Figure Lengend Snippet: Restoration of functionality of exhausted CD4 cells with IL-7. PBMC from 40 donors were tested in an ELISPOT assay with 50 μg/mL of HCMV antigen, with and without IL-7 added to the culture. Cytokine recall response for IFN-γ, IL-2, IL-4, and IL-17 was recorded as SFU. PBMC were plated at 250,000 cells and the concentration of IL-7 was 30 ng/mL. The SFU with and without IL-7 for each of the cytokines is shown.

Article Snippet: The 96-well ELISPOT assays were performed using human interferon-γ ImmunoSpot ® kit (CTL-HIFNG-1/5M), IL-2 ImmunoSpot ® kit (CTL-HIL2-1/5), IL-4 ImmunoSpot ® kit (HIL4-1/5) and IL-17 ImmunoSpot ® kit (CTL-HIL17-1/5) as well as the IFN-γ /IL-2 double-color enzymatic kit (CTL-HIFNGIL2-1/5) from Cellular Technology Ltd. Plates and all antibodies, tertiaries, diluents, and substrate are contained in the kits.

Techniques: Enzyme-linked Immunospot, Concentration Assay

Effects of tumour necrosis factor (TNF)-α blockade on the frequencies of antigen-specific Th1/Th17 cells in spleen and central nervous system (CNS). (a) Results of enzyme-linked immunospot (elispot) assays in phosphate-buffered saline (PBS) (n = 12)-, Humira® (n = 8)- and Enbrel®-treated mice (n = 8) for interferon (IFN)-γ (**P < 0·01, rank sum test) and interleukin (IL-17 (*P < 0·05, rank sum test) in the spleen. (b) Results of IFN-γ/IL-17 elispot assays in PBS (n = 13)-, Humira® (n = 5)- and Enbrel®-treated mice (n = 8 IFN-γ; n = 7 IL-17) in the spinal cord.

Journal: Clinical and Experimental Immunology

Article Title: Blockade of tumour necrosis factor-α in experimental autoimmune encephalomyelitis reveals differential effects on the antigen-specific immune response and central nervous system histopathology

doi: 10.1111/cei.12209

Figure Lengend Snippet: Effects of tumour necrosis factor (TNF)-α blockade on the frequencies of antigen-specific Th1/Th17 cells in spleen and central nervous system (CNS). (a) Results of enzyme-linked immunospot (elispot) assays in phosphate-buffered saline (PBS) (n = 12)-, Humira® (n = 8)- and Enbrel®-treated mice (n = 8) for interferon (IFN)-γ (**P < 0·01, rank sum test) and interleukin (IL-17 (*P < 0·05, rank sum test) in the spleen. (b) Results of IFN-γ/IL-17 elispot assays in PBS (n = 13)-, Humira® (n = 5)- and Enbrel®-treated mice (n = 8 IFN-γ; n = 7 IL-17) in the spinal cord.

Article Snippet: However, we observed no difference in the antigen-specific Th1/Th17 response with Enbrel® treatment in the spinal cord (Fig. b). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 Effects of tumour necrosis factor (TNF)-α blockade on the frequencies of antigen-specific Th1/Th17 cells in spleen and central nervous system (CNS). (a) Results of enzyme-linked immunospot ( elispot ) assays in phosphate-buffered saline (PBS) ( n = 12)-, Humira® ( n = 8)- and Enbrel®-treated mice ( n = 8) for interferon (IFN)-γ (** P < 0·01, rank sum test) and interleukin (IL-17 (* P < 0·05, rank sum test) in the spleen. (b) Results of IFN-γ/IL-17 elispot assays in PBS ( n = 13)-, Humira® ( n = 5)- and Enbrel®-treated mice ( n = 8 IFN-γ; n = 7 IL-17) in the spinal cord.

Techniques: Enzyme-linked Immunospot